United States Patent  5,235,041
Cappello, et. al. Aug. 10, 1993
Purification of structurally ordered recombinant protein polymers
Inventors:  Cappello; Joseph (San Diego, CA); Ferrari; Franco A. (La Jolla, CA); Buerkle; Tina L. (Solano Beach, CA); Textor; Garret (San Diego, CA). 
Assignee:  Protein Polymer Technologies, Inc. (San Diego, CA). 
Appl. No.:  869,212
Filed:  Apr. 13, 1992
Related U.S. Application Data
Continuation of (including streamline cont.) Ser. No. 635,463, Dec. 28, 1990, abandoned.
 
Intl. Cl. :  C07K 3/12
Current U.S. Cl.:  530/353; 530/418; 530/420; 530/422; 530/423
Field of Search:  530/353, 354, 355, 356, 357, 370, 412, 418, 420, 427, 422, 423
References Cited | [Referenced By]
U.S. Patent Documents
4,511,502 Apr., 1985 Builder et al. 530/344
4,512,922 Apr., 1985 Jones et al. 424/85.8
4,518,526 May, 1985 Olson 530/344
4,620,948 Nov., 1986 Builder et al. 530/419
4,652,630 Mar., 1982 Bentle et al. 530/344
4,766,205 Aug., 1988 Ghosh-Dashidar 530/412
Foreign Patent Documents
8704726 Aug., 1987 WO
 
Other References

Marston, Biochem. J., 240, pp. 1-12 (1986).

Kane et al., Trend-Biotech., vol. 6, pp. 95-101 (1988).

Marston et al., Biotechnology, vol. 2 pp. 799-804 (1984).

Primary Examiner: Russel; Jeffrey E.
Attorney, Agent or Firm: Rowland; Bertram I.

Abstract

Recombinant structural and functional polymers are purified by lysing of the cellular host, separation of the solid materials, washing and extraction of contaminants using a detergent solution at elevated temperatures.

6 Claims, No Drawings


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